Contemporary Enzyme-Based Methods for Recombinant Proteins In Vitro Phosphorylation

Phosphorylation is a reversible, enzyme-controlled posttranslational process affecting approximately one-third of all proteins in eukaryotic cells at any given time. Any deviation the degree and/or site phosphorylation leads to an abnormal conformation proteins, resulting decline or loss their function. Knowledge phosphorylation-related pathways essential for understanding disease pathogenesis and design new therapeutic strategies. Recent availability various kinases affordable price differs activity, specificity, stability provides opportunity studying modulating this reaction vitro. We can exploit knowledge other applications. There enormous potential produce fully decorated active recombinant either biomedical cosmetic Closely related possibility current achievements develop safe efficacious vaccines, drugs, immunomodulators. In review, we outlined enzyme-based possibilities vitro peptides added value that immobilized provide.